研究大蜡螟幼虫的细菌感染：深入了解病原体传播和行为
Journal of Invertebrate Pathology
(
IF
2.4
)
Pub Date : 2023-08-02
, DOI:
10.1016/j.jip.2023.107975
Joana Admella
1
,
Eduard Torrents
1
Affiliation
Bacterial Infections and Antimicrobial Therapies Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Baldiri Reixac 15-21, 08028 Barcelona, Spain; Microbiology Section, Department of Genetics, Microbiology and Statistics, Faculty of Biology, University of Barcelona, 643 Diagonal Ave., 08028 Barcelona, Spain.
大蜡螟昆虫是一种广泛用于研究细菌感染的替代动物模型。它具有广泛的优势，包括成本低、易于维护且不受道德约束。除其他特征外，它们的先天免疫系统与哺乳动物非常相似。在这项研究中，我们解剖了几种感染重要人类病原体的幼虫：脓肿分枝杆菌、金黄色葡萄球菌和铜绿假单胞菌。通过在显微镜下观察脂肪体、肠道、气管和血淋巴，我们能够描述细菌倾向于传播的地方。我们还量化了整个感染过程中血淋巴中的细菌数量，发现不同病原体之间存在显着差异。通过这项工作，我们旨在更好地了解受感染幼虫中细菌的行为和传播。
"点击查看英文标题和摘要"
Investigating bacterial infections in Galleria mellonella larvae: Insights into pathogen dissemination and behavior
The insect Galleria mellonella is an alternative animal model widely used for studying bacterial infections. It presents a wide range of advantages, including its low cost, easy maintenance and lack of ethical constraints. Among other features, their innate immune system is very similar to that of mammals. In this study, we dissected several larvae infected with important human pathogens: Mycobacterium abscessus, Staphylococcus aureus and Pseudomonas aeruginosa. By observing the fat body, gut, trachea, and hemolymph under the microscope, we were able to describe where bacteria tend to disseminate. We also quantified the number of bacteria in the hemolymph throughout the infection course and found significant differences between the different pathogens. With this work, we aimed to better understand the behavior and dissemination of bacteria in the infected larvae.
更新日期：2023-08-02